Cytochemical and morphologic identification of macrophages and eosinophils in tissue cultures of normal human bone marrow.

When normal human bone marrow cells logic and cytochemical properties. The are cultured in soft agar, in the presence of identification of macrophage-monocytes human spleen-conditioned medium, two and of eosinophils was based on light, types of colonies could be recognized by phase-contrast, and electron microscopy, means of the inverted microscope: large the strong positive reaction of a-naphthyl colonies consisting of macrophagesacetate esterase in the macrophagemonocytes, and small colonies consisting monocyte, and the positive stain with of eosinophils. In the two types of colonies, Luxol Fast Blue, eosin, and peroxidase of cells developing from blasts to mature the eosinophils. cells could be recognized by their morpho-